ON THE NATURE OF DIPHTHERIA TOXIN

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On the Nature of Diphtheria Toxin

The chemical nature and the formation of bacterial toxins has been widely discussed. Diphtheria toxin may be regarded as a classical subject. Most of the earlier authors regarded it as a protein or a product derived therefrom. Recently some American authors, Warden, Connell, and Holly, have emphatically declared the toxin to be a "colloidal" mixture of fats and protein. As to the formation of t...

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The Production of Diphtheria Toxin

Toxin of sufficient strength to kill a 400-gramme guinea-pig in three days and a half in a dose of 0.cubic centimetre developed in suitable bouillon, contained in ordinary Erlenmeyer flasks, within a period of twenty-four hours. In such boullon the toxin reached its greatest strength in from four to seven days (0.005 cubic centimetre killing a 500-gramme guinea-pig in three days). This period o...

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Diphtheria Toxin In

Recent studies have demonstrated that diphtheria toxin is an enzyme of unusual type. Small amounts of the toxin, added to nicotinamide adenine dinucleotide (NAD)-containing mammalian cell extracts, block peptide-bond formation by catalyzing inactivation of the translocating enzyme, aminoacyltransferase 2 (T2) (1-3). This highly specific reaction involves the splitting of NAD with liberation of ...

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The Effects of Diphtheria Toxin on the Cecropia Silkworm

1. The metamorphosis of the Cecropia silkworm is accompanied by large and systematic changes in the insect's sensitivity to diphtheria toxin. 2. Injection of less than 1 gamma of toxin into mature caterpillars, prepupae, or developing adults causes cessation of development followed by delayed death 1 to 5 weeks later. 3. Dormant pupae, on the contrary, are resistant to 70 gamma of toxin and may...

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ژورنال

عنوان ژورنال: Journal of General Physiology

سال: 1926

ISSN: 1540-7748,0022-1295

DOI: 10.1085/jgp.8.4.311